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Clone & Sequence Service
Features and Benefits
- Strong expertise in PCR design and testing
- Purification and cloning of PCR fragments in E.coli (blunt-end or TA-overhang approach)
- Selection and picking of the desired amount of clones
- Sanger sequencing and detailed reporting
- Shipment of the selected clones and/or plasmids (up to transfection grade, scalable up to 10 mg)
Service
PCR amplification of heterogeneous biological samples results in a heterogeneous mixture of PCR products (e.g. ribosomal 16S PCR generated from stool or soil samples). In order to be able to sequence individual PCR products by DNA Sanger sequencing, it is first necessary to clone them. At Microsynth, we are able to perform the entire process of cloning and sequencing, no matter how many clones your sequencing project may require. If you need the plasmids back Microsynth will carry out the DNA isolation for you.
Best-approach consulting
Beyond Sanger DNA sequencing, we are also able to offer you comprehensive next generation sequencing services. In general, a next generation sequencing approach allows circumventing the cloning step and becomes economical with increasing number of samples and throughput requirements. Please contact our experts to figure out which approach best fits your needs.