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DNA Oligonucleotides

DNA Oligonucleotides
 
 
Microsynth uses proprietary synthesizer technologies to offer DNA oligonucleotides of the highest quality. Our state-of-the-art production facilities enable fast synthesis of standard primers, modified and functionalized oligonucleotides as well as various PCR probes.
 

Features and Benefits

 
 
Highest Quality
  • Stringent quality control (online Trityl monitoring and MALDI-TOF MS or analytical PAGE)
  • Microsynth is a pioneer with a successful track record of over 30 years
 
Fast
  • Desalted unmodified oligos up to 1 µmol synthesis scale are shipped the next day
  • Purified unmodified oligos up to 1 µmol synthesis scale are shipped within 2 days
  • Standard modified oligos are shipped within 3 days
 
Comprehensive Service Portfolio
  • Large range of synthesis scales (yields from nmol to µmol are available)
  • Broad range of 5’-, 3’- and internal modifications
  • 4 different purity levels
 
 
 
Cost Effective
  • Very competitive pricing
  • High guaranteed yields

 

Excellent Technical Support
  • Our highly experienced and well-trained scientists are happy to support you
 
User-friendly Online Ordering System
  • Easy-to-use online portal with a series of helpful tools (e.g. order tracking & history, convenient search and re-order option)

 

Synthesis Scales

 
Microsynth is offering its DNA oligos at 5 different scales.
The available standard scales are Genomics, 0.04 µmol, 0.2 µmol, 1.0 µmol and 15 µmol.
As the yield and length restrictions depend also on the purification used please have a look at the tables below.
 
 
 
Desalted DNA Oligos
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics 13 - 60 2 10 1
0.04 µmol 13 - 80 3 15 1
0.2 µmol 6 - 1504 10 50 1
1.0 µmol 6 - 80 50 250 1
15 µmol 13 -60 700 3'500 2
 
RP-HPLC Purified DNA Oligos 
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics not available
0.04 µmol 13 - 50 1 5 2
0.2 µmol 6 - 50 3 15 2
1.0 µmol 6 - 50 15 75 2
15 µmol 6 - 50 300 1'500 3
 
IEX-HPLC Purified DNA Oligos 
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]5
Genomics not available
0.04 µmol not available
0.2 µmol 40 - 80 2 3.3 3-5
1.0 µmol 6 10 3-5
15 µmol not available
 
RP-HPLC Purified & Dialyzed DNA Oligos 
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics not available
0.04 µmol not available
0.2 µmol 8 - 50 3 15 3
1.0 µmol 8 - 50 15 75 3
15 µmol 8 - 50 200 1'000 4
 
PAGE Purified DNA Oligos
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics not available
0.04 µmol 13 - 80 0.5 2.5 2
0.2 µmol

8 - 80

81 - 1504

2

0.5

10

2.5

 2
1.0 µmol 8 - 80 7 35 2
15 µmol not available
 
 
1 The synthesis scale represents the initial amount of 3' bases (starting material).
2 Our guaranteed and average yields are measured in OD and are valid only for unmodified oligos >20mer.
3 Yields indicated in nmol represent an example calculation for a 20mer. For this calculation the following rule of thumb equation was applied: nmol of oligo = OD x 100/length of oligo. Please note that this calculation is based on sequences with virtually homogenous distribution of the 4 DNA bases; it may vary for sequences with high GC contents >70% etc.
4 Oligos longer than 150 DNA bases on request (we would like to discuss the proposed experiment/application with you beforehand in order to guarantee the best possible outcome)
5 Yields indicated in nmol represent an example calculation for a 60 nt DNA Oligo. Formula: nmol= OD*100/length of the Oligo

Modifications

 

Microsynth offers a wide variety of modifications (5’, 3’ and internal) for DNA oligonucleotides. To give you a better overview, they have been categorized as: 

To learn more about the key specifications of a modification, please look at Modifications.

 

If your desired modification is not listed please contact us for availability.

How to Order

 
  • Enter our webshop
  • Click on DNA in the "DNA/RNA Synthesis" domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering )
  • Follow the further instructions

 

To obtain information about our prices, please login to our webshop and download the current price list or ask your sales manager for a specific quote (attractive discounts are available if you plan to order more regularly from Microsynth or if you have a volume order).